CBR1 ELISA Kit (Pig) (OKEH07556)

Catalog No: OKEH07556

Size:96T

Pig CBR1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	98-110% (n = 20)	85-97% (n = 20)	98-109% (n = 20)	105-114% (n = 20)
  EDTA plasma(n=5)	105-113% (n = 20)	90-102% (n = 20)	102-112% (n = 20)	104-113% (n = 20)
  heparin plasma(n=5)	82-90% (n = 20)	104-116% (n = 20)	94-103% (n = 20)	100-109% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CBR1 ELISA Kit (Pig) (OKEH07556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CBR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CBR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CBR1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.7% (n = 20)
  Mean Inter-assay CV%: <=10.1% (n = 20)
• ELISA Kit Component:
  • CBR1 Microplate: 96 Wells (12 x 8 Well strips)
  • CBR1 Lyophilized Standard: 2
  • 100X Biotinylated CBR1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (By similarity).
  Subcellular Location: Cytoplasm
  Unigene: Ssc.55707
  SMR: Q28960
  KEGG: ssc:397143
  Pfam: PF00106
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.21ng/mL
• Specificity: Natural and recombinant Pig Carbonyl reductase [NADPH] 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CBR1
• Gene Full Name: carbonyl reductase 1
• Alias Symbols: 15-hydroxyprostaglandin dehydrogenase;15-hydroxyprostaglandin dehydrogenase [NADP(+)];20-beta-hydroxysteroid dehydrogenase;carbonyl reductase [NADPH] 1;NADPH-dependent carbonyl reductase 1;PG-9-KR;prostaglandin 9-ketoreductase;prostaglandin-E(2) 9-reductase.
• NCBI Gene Id: 397143
• Protein Name: Q28960
• Description of Target: Carbonyl reductase [NADPH] 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q28960
• Protein Accession #: NP_999238.1
• Nucleotide Accession #: NM_214073.1