Cbl Antibody (OAPB00462)

Catalog No: OAPB00462

Size:100UG

Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Cbl Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot
• Reconstitution and Storage: Cbl antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl. The immunogen is located within amino acids 810 - 860 of Cbl.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Cbl antibody is Catalog # OEPB02572
• Application Info: WB 0.5~2 ug/ml
  IF 20 ug/ml
• Other Applications Image 1 Data: Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 ug/mL.
• Reference: Thien CBF and Langdon WY. C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses. Biochem. J. 2005; 391:153-66;Weissman AM. Themes and variations on ubiquitylation. Nat. Rev. Mol. Cell Biol. 2001; 2:169-78.;Swaminathan G and Tsygankov AY. The Cbl family of proteins: ring leaders in regulation of cell signaling. J. Cell. Physiol. 2006; 209:21-43.;

Target Info

• Gene Symbol: CBL
• Gene Full Name: Cbl proto-oncogene
• Alias Symbols: Cas-Br-M (murine) ecotropic retroviral transforming sequence;casitas B-lineage lymphoma proto-oncogene;Cbl proto-oncogene, E3 ubiquitin protein ligase;CBL2;C-CBL;E3 ubiquitin-protein ligase CBL;FRA11B;fragile site, folic acid type, rare, fra(11)(q23.3);NSLL;oncogene CBL2;proto-oncogene c-Cbl;RING finger protein 55;RING-type E3 ubiquitin transferase CBL;RNF55;signal transduction protein CBL.
• NCBI Gene Id: 867
• Protein Name: E3 ubiquitin-protein ligase CBL
• Description of Target: Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
• Uniprot ID: P22681
• Protein Accession #: P22681