CASP6 Recombinant Protein (Human) (OPED00019)

Catalog No: OPED00019

Size:5000U

SDS-PAGE Analysis - Lane 1: MW Marker, Lane 2: 1 ug Caspase-6.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid 50mM HEPES (pH 7.4), containing 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM DTT
• Host: E. Coli
• Reconstitution and Storage: Store at -80°C. Avoid repeated freeze/thaw cycles. After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
• Purity: >80% (SDS-PAGE)
• Biological Activity: One U=1 pmol/min at 30°C using Ac-VEID-pNA (200mM; Prod. No. BML-P437) as substrate. Activity: 100 U/ul
• Application Info: Useful tool to study enzyme regulation and kinetics, cleave target substrates, screen for inhibitors.
• Reference: Product Literature References
  Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment: J. Gafni, et al.; J. Neurosci 32, 7454 (2012), Abstract;
  Induction of the C-terminal proteolytic cleavage of Abeta PP by statins: O. Descamps, et al.; J. Alzheimers Dis. 25, 51 (2011), Abstract;
  Identification and Evaluation of Novel Small Molecule Pan-Caspase Inhibitors in Huntington’s Disease Models: M.J. Leyva, et al.; Chem. Biol. 17, 1189 (2010), Abstract;
  General Literature References
  Caspase-mediated cleavage of DNA topoisomerase I at unconventional sites during apoptosis: K. Samejima, et al.; J. Biol. Chem. 274, 4335 (1999), Abstract;
  Biochemical characteristics of caspases-3, -6, -7, and -8: H.R. Stennicke & G.S. Salvesen; J. Biol. Chem. 272, 25719 (1997), Abstract;
  Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells: L. Faleiro, et al.; EMBO J. 16, 2271 (1997), Abstract;
  Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta -converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis: A. Takahashi, et al.; PNAS 93, 8395 (1996), Abstract;
  The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A: K. Orth, et al.; J. Biol. Chem. 271, 16443 (1996), Abstract;
  Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family: T. Fernandes-Alnemri, et al.; Cancer Res 55, 2737 (1995), Abstract;

Target Info

• Gene Symbol: CASP6
• Gene Full Name: caspase 6
• Alias Symbols: apoptotic protease MCH-2;caspase 6, apoptosis-related cysteine peptidase;caspase 6, apoptosis-related cysteine protease;caspase-6;MCH2.
• NCBI Gene Id: 839
• Protein Name: Caspase-6
• Description of Target: Effector caspase responsible for the cleavage of nuclear lamins in apoptosis. Caspase-6 cleavages of various other protein targets have been implicated in the pathogenesis of neurodegenerative diseases, including Alzheimer’s disease (b-amyloid precursor protein), Huntington’s disease (huntingtin).
• Uniprot ID: P55212
• Protein Accession #: NP_004350.1
• Nucleotide Accession #: NM_004359.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 18 kDa +11 kDa