CAPN2 ELISA Kit (Pig) (OKEH08505)

Catalog No: OKEH08505

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	98-111% (n = 20)	110-120% (n = 20)	106-115% (n = 20)	86-95% (n = 20)
  EDTA plasma(n=5)	100-110% (n = 20)	102-113% (n = 20)	96-109% (n = 20)	101-111% (n = 20)
  heparin plasma(n=5)	104-116% (n = 20)	102-113% (n = 20)	92-102% (n = 20)	96-106% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CAPN2 ELISA Kit (Pig) (OKEH08505) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPN2 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.5% (n = 20)
  Mean Inter-assay CV%: <=7.9% (n = 20)
• ELISA Kit Component:
  • CAPN2 Microplate: 96 Wells (12 x 8 Well strips)
  • CAPN2 Lyophilized Standard: 2
  • 100X Biotinylated CAPN2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
  Subcellular Location: Cytoplasm Cell membrane Translocates to the plasma membrane upon Ca(2+) binding.
  Unigene: Ssc.49836
  SMR: P43367
  Pfam: PF01067
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Calpain-2 catalytic subunit
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CAPN2
• Alias Symbols: Calcium-activated neutral proteinase 2;Calpain M-type;Calpain-2 large subunit;Millimolar-calpain.
• Protein Name: P43367
• Description of Target: Calpain-2 catalytic subunit
• Uniprot ID: https://www.uniprot.org/uniprot/P43367