CALM ELISA Kit (Rabbit) (OKWB00402)

Catalog No: OKWB00402

Size:96T

Rabbit CALM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-102%	85-95%	91-101%	87-101%
  EDTA Plasma (n=5)	82-98%	85-100%	84-99%	84-100%
  Heparin Plasma (n=5)	87-97%	81-96%	81-90%	88-100%

• ELISA Kit Principle: Aviva Systems Biology Calm ELISA Kit (Rabbit) (OKWB00402) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calm has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Calm is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calm captured in the well.
• ELISA Kit Range: 78.125-5000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	88-97	91
  EDTA Plasma (n=5)	85-105	94
  Heparin Plasma (n=5)	88-105	96

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Calm Microplate: 96 Wells (12 x 8 Well strips)
  • Calm Lyophilized Standard: 2 x 5 ng
  • 100X Calm Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 46.9 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CALM1
• Gene Full Name: calmodulin 1
• Alias Symbols: calmodulin.
• NCBI Gene Id: 100350107
• Protein Name: Calmodulin
• Description of Target: Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity).
• Uniprot ID: P62160
• Protein Accession #: NP_001182569
• Nucleotide Accession #: NM_001195640