C1QL3 ELISA Kit (Human) (OKCA00643)

Catalog No: OKCA00643

Size:96 Wells

Human C1QL3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	105	100-110
  1:2	99	95-107
  1:4	89	85-93
  1:8	84	80-88

• ELISA Kit Principle: Aviva Systems Biology C1QL3 ELISA Kit (Human) (OKCA00643) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1QL3 captured in the well.
• ELISA Kit Range: 0.312 ng/mL-20 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	90-100
  EDTA Plasma (n=4)	90	85-95

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level C1QL3 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level C1QL3 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • C1QL3 Microplate: 96 Wells (12 x 8 Well strips)
  • C1QL3 Lyophilized Standard: 2
  • 100X Biotinylated C1QL3 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1QL3
• Gene Full Name: Complement C1q like 3
• Alias Symbols: C1ql, K100, CTRP13, C1QTNF13
• NCBI Gene Id: 389941
• Protein Name: Complement C1q-like protein 3
• Description of Target: May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Plays a role in glucose homeostasis. Via AMPK signaling pathway, stimulates glucose uptake in adipocytes, myotubes and hepatocytes and enhances insulin-stimulated glucose uptake. In a hepatoma cell line, reduces the expression of gluconeogenic enzymes G6PC and PCK1 and hence decreases de novo glucose production.
• Uniprot ID: Q5VWW1
• Protein Accession #: NP_001010908.1
• Nucleotide Accession #: NM_001010908.1