C-JUN Protein (OPPA01181)

Catalog No: OPPA01181 (Formerly GWB-8D9488)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: C-JUN is supplied as lyophilized powder containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.
  Biological Activity: C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
  Product Note: Kinase activity may vary depending on the substrate and reaction conditions.
  Product Introduction: C-JUN is a gene which, in combination with c-Fos, forms the AP-1early response transcription factor. It is activated by the JNKpathway.This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
  Product Description: C-JUN amino acids 1-81 produced in E.coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa.C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 1-2 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Purity: Greater than 95% as determined by SDS-PAGE.

Target Info

• Gene Symbol: C-JUN
• Alias Symbols: AP1, p39, AP-1, cJUN, c-Jun
• NCBI Gene Id: 3725
• Protein Name: Transcription factor AP-1
• Description of Target: Recombinant C-JUN
• Uniprot ID: P05412
• Protein Accession #: NP_002219.1
• Protein Size (# AA): Recombinant