BLVRB ELISA Kit (Human) (OKCD02004)

Catalog No: OKCD02004 (Formerly GWB-KBBLQ8)

Size:96WELLS

Human BLVRB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase B (BLVRB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase B (BLVRB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase B (BLVRB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Biliverdin Reductase B (BLVRB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biliverdin Reductase B (BLVRB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biliverdin Reductase B (BLVRB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CILP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CILP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CILP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.122 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase B (BLVRB). No significant cross-reactivity or interference between Biliverdin Reductase B (BLVRB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BLVRB
• Gene Full Name: biliverdin reductase B
• Alias Symbols: Biliverdin reductase B;biliverdin-IX beta-reductase;BVRB;BVR-B;epididymis secretory protein Li 10;flavin reductase (NADPH);FLR;FR;GHBP;green heme-binding protein;HEL-S-10;NADPH-dependent diaphorase;NADPH-flavin reductase;SDR43U1;short chain dehydrogenase/reductase family 43U, member 1.
• NCBI Gene Id: 645
• Protein Name: Flavin reductase (NADPH)
• Description of Target: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
• Uniprot ID: P30043
• Protein Accession #: NP_000704.1
• Nucleotide Accession #: NM_000713.2
• Protein Size (# AA): 206