Biopterin ELISA Kit (OKEH02614)

Catalog No: OKEH02614

Size:96T

Biopterin ELISA Kit (OKEH02614) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Biopterin ELISA Kit (OKEH02614) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Biopterin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Biopterin and incubated. The Biopterin found in the sample or standards competes with the biotinylated Biopterin for limited binding sites on the immobilized anti-Biopterin antibody. Excess unbound biotinylated Biopterin and sample or standard Biopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Biopterin captured in the well and inversely proportional to the amount of Biopterin which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200nmol/L
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.6% (n = 20)
  Mean Inter-assay CV%: < 10.5% (n = 20)
• ELISA Kit Component:
  • Anti-Biopterin Microplate: 96 Wells (12 x 8 Well strips)
  • Biopterin Lyophilized Standard: 2 x 200 nmol
  • Sample Diluent: 1 x 20 mL
  • 100X Biopterin-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: Biopterin
  Target Name: Biopterin
  Pubchem: 2380
  Chemical formula: C9H11N5O3
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 1.23nmol/L
• Specificity: Natural and recombinant General Biopterin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: Biopterin, Biopterin, Biopterin
• Protein Name: Biopterin
• Description of Target: Biopterin is a coenzyme that is produced within the body. It is an oxidized degradation product of tetrahydrobiopterin. Defects in biopterin synthesis or regeneration can cause a form of hyperphenylalaninemia (a disease with symptoms similar to PKU). Biopterin is synthesized in several parts of the body, including the pineal gland. Biopterin deficiency has been associated with a variety of disorders, including dystonia and rabies.