BHMT2 ELISA Kit (Human) (OKEH08230)

Catalog No: OKEH08230

Size:96T

Human BHMT2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology BHMT2 ELISA Kit (Human) (OKEH08230) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BHMT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BHMT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BHMT2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.8% (n = 20)
  Mean Inter-assay CV%: <=10.1% (n = 20)
• ELISA Kit Component:
  • BHMT2 Microplate: 96 Wells (12 x 8 Well strips)
  • BHMT2 Lyophilized Standard: 2
  • 100X Biotinylated BHMT2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in the regulation of homocysteine metabolism. Converts homocysteine to methionine using S-methylmethionine (SMM) as a methyl donor.
  Unigene: Hs.114172
  SMR: Q9H2M3
  String: 9606.ENSP00000255192
  KEGG: hsa:23743
  MIM: 605932
  Pfam: PF02574
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.069ng/mL
• Specificity: Natural and recombinant Human S-methylmethionine--homocysteine S-methyltransferase BHMT2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BHMT2
• Gene Full Name: betaine--homocysteine S-methyltransferase 2
• Alias Symbols: betaine-homocysteine methyltransferase 2;Betaine--homocysteine S-methyltransferase 2;S-methylmethionine--homocysteine S-methyltransferase BHMT2;SMM-hcy methyltransferase.
• NCBI Gene Id: 23743
• Protein Name: Q9H2M3
• Description of Target: S-methylmethionine--homocysteine S-methyltransferase BHMT2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H2M3
• Protein Accession #: NP_001171476.1
• Nucleotide Accession #: NM_001178005.1