BHMT ELISA Kit (Bovine) (OKEH07939)

Catalog No: OKEH07939

Size:96T

Bovine BHMT ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology BHMT ELISA Kit (Bovine) (OKEH07939) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BHMT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BHMT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BHMT captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.3% (n = 20)
  Mean Inter-assay CV%: <=6.5% (n = 20)
• ELISA Kit Component:
  • BHMT Microplate: 96 Wells (12 x 8 Well strips)
  • BHMT Lyophilized Standard: 2
  • 100X Biotinylated BHMT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  Subcellular Location: Cytoplasm
  Unigene: Bt.16399
  SMR: Q5I597
  String: 9913.ENSBTAP00000002916
  KEGG: bta:497025
  Pfam: PF02574
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.09ng/mL
• Specificity: Natural and recombinant Bovine Betaine--homocysteine S-methyltransferase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BHMT
• Gene Full Name: betaine--homocysteine S-methyltransferase
• Alias Symbols: betaine-homocysteine methyltransferase;betaine--homocysteine S-methyltransferase 1.
• NCBI Gene Id: 497025
• Protein Name: Q5I597
• Description of Target: Betaine--homocysteine S-methyltransferase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q5I597
• Protein Accession #: NP_001011679.1
• Nucleotide Accession #: NM_001011679.1