BCKDHB Antibody - N-terminal region (OAAB00689)

Catalog No: OAAB00689 (Formerly GWB-DP47F6)

Size:400ul

BCKDHB Antibody (N-term) (Cat. #OAAB00689) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).

Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) (Cat. #OAAB00689). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.

BCKDHB Antibody (N-term) (Cat. #OAAB00689) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of BCKDHB Antibody (N-term)(Cat#OAAB00689) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

BCKDHB Antibody (N-term) (Cat. #OAAB00689) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P-Leica, IF, FC
• Additional Information: Function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  Cellular Location: Mitochondrion matrix.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IF: 1:25
  IHC-P-Leica: 1:500
  FC: 1:10~50
• Description:
  View product citations for BCKDHB Antibody - N-terminal region (OAAB00689) on CiteAb

Target Info

• Gene Symbol: BCKDHB
• Alias Symbols: E1B, BCKDE1B, BCKDH E1-beta
• NCBI Gene Id: 594
• Protein Name: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
• Description of Target: Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.
  
• Uniprot ID: P21953
• Protein Accession #: NP_000047.1
• Molecular Weight: 43 kDa