BATF Antibody (OAAF02470)

Catalog No: OAAF02470 (Formerly GWB-ASD699)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BATF Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BATF.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: BATF antibody detects endogenous levels of total BATF protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Dorsey M.J., Oncogene 11:2255-2265(1995).
  Hasegawa H., Biochem. Biophys. Res. Commun. 222:164-170(1996).
  Meyer N.P., Mamm. Genome 9:849-852(1998).

Target Info

• Gene Symbol: BATF
• Gene Full Name: basic leucine zipper ATF-like transcription factor
• Alias Symbols: activating transcription factor B;basic leucine zipper transcription factor, ATF-like;basic leucine zipper transcriptional factor ATF-like;B-ATF;BATF1;B-cell-activating transcription factor;SFA2;SFA-2;SF-HT-activated gene 2 protein.
• NCBI Gene Id: 10538
• Protein Name: Basic leucine zipper transcriptional factor ATF-like
• Description of Target: AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs (By similarity).
• Uniprot ID: Q16520
• Protein Accession #: NP_006390.1
• Molecular Weight: 14 kDa