BACE Antibody (OAPB00100)

Catalog No: OAPB00100 (Formerly GWB-980BE2)

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Western blot analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide (2253P) and in mouse 3T3 cell lysate (C) with BACE antibody at 1 μg/mL.

Immunohistochemistry of BACE in mouse brain tissue with BACE antibody at 2.5 ug/ml.

Immunocytochemistry of BACE in 3T3 cells with BACE antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Bovine|Guinea Pig|Human|Mouse|Rat
• Product Format: Liquid. BACE Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Brain Tissue Lysate, 3T3 Cell Lysate (OCPB00170)
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: BACE antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-BACE antibody (2253) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BACE antibody is Catalog # OEPB02226
• Application Info: BACE can be used for detection of BACE by Western blot at 0.5 to 1 ug/ml. An approximately 70 kDa band should be detected. (Optimal dilution should be determined by user)
• Reference: Vassar R, Bennett BD, Babu-Khan S, et al. beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41 ;Hussain I, Powell D, Howlett DR , et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci 1999;14:419-27 ;Yan R, Bienkowski MJ, Shuck ME, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999;402:533-7;Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999;402:537-40 (WD0500)

Target Info

• Gene Symbol: BACE1
• Gene Full Name: beta-secretase 1
• Alias Symbols: APP beta-secretase;asp 2;ASP2;aspartyl protease 2;BACE;beta-secretase 1;beta-secretase 1 precursor variant 1;beta-site amyloid beta A4 precursor protein-cleaving enzyme;Beta-site amyloid precursor protein cleaving enzyme 1;beta-site APP cleaving enzyme 1;beta-site APP-cleaving enzyme;HSPC104;memapsin-2;membrane-associated aspartic protease 2;transmembrane aspartic proteinase Asp2.
• NCBI Gene Id: 23621
• Protein Name: Beta-secretase 1|Homo sapiens beta-site APP cleaving enzyme (BACE) mRNA, complete cds
• Description of Target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
• Uniprot ID: P56817
• Protein Accession #: AF190725
• Nucleotide Accession #: https://www.ncbi.nlm.nih.gov/nuccore/AF190725
• Molecular Weight: Predicted: 55kD Observed: 65kD (Post-modification: 4 N-linked glycosylation)