AZGP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03314)

Catalog No: OKCD03314

Size:96WELLS

Human AZGP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-105%	91-99%	84-103%	92-101%
  EDTA Plasma (n=5)	98-105%	88-101%	92-99%	80-91%
  Heparin Plasma (n=5)	78-97%	98-105%	79-94%	87-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) level in the sample or standard.
• ELISA Kit Range: 0.82-600ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-98	89
  EDTA Plasma (n=5)	85-104	92
  Heparin Plasma (n=5)	84-105	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AZGP1 Microplate: 96 Wells (12 x 8 Well strips)
  • AZGP1 Lyophilized Standard: 2
  • 100X AZGP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, saliva, seminal plasma, sweat and other biological fluids
• Sensitivity: 0.82 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AZGP1
• Gene Full Name: alpha-2-glycoprotein 1, zinc-binding
• Alias Symbols: Alpha-2-glycoprotein, zinc;testicular tissue protein Li 227;ZA2G;ZAG;zinc-alpha-2-glycoprotein;zn-alpha-2-glycoprotein;Zn-alpha2-glycoprotein;zn-alpha-2-GP.
• NCBI Gene Id: 563
• Protein Name: Zinc-alpha-2-glycoprotein
• Description of Target: Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
• Uniprot ID: P25311
• Protein Accession #: NP_001176
• Nucleotide Accession #: NM_001185