ATP2A3 ELISA Kit (Chicken) (OKEH08750)

Catalog No: OKEH08750

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ATP2A3 ELISA Kit (Chicken) (OKEH08750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP2A3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP2A3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP2A3 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.4% (n = 20)
  Mean Inter-assay CV%: <=6.3% (n = 20)
• ELISA Kit Component:
  • ATP2A3 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP2A3 Lyophilized Standard: 2
  • 100X Biotinylated ATP2A3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  Subcellular Location: Endoplasmic reticulum membrane Multi-pass membrane protein Sarcoplasmic reticulum membrane Multi-pass membrane protein
  Unigene: Gga.16523
  String: 9031.ENSGALP00000002373
  KEGG: gga:395707
  Pfam: PF00689
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATP2A3
• Gene Full Name: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3
• Alias Symbols: ATPase, Ca++ transporting, ubiquitous;calcium pump 3;chkSERCA3;sarcoendoplasmic reticulum calcium ATPase;sarcoplasmic/endoplasmic reticulum calcium ATPase 3;SERCA;SERCA3;SR Ca(2+)-ATPase 3.
• NCBI Gene Id: 395707
• Protein Name: Q9YGL9
• Description of Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q9YGL9
• Protein Accession #: NP_990222.1
• Nucleotide Accession #: NM_204891.1