ATF2 ELISA Kit (Rat) (OKEH03723)

Catalog No: OKEH03723

Size:96T

Rat ATF2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATF2 ELISA Kit (Rat) (OKEH03723) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atf2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Atf2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atf2 captured in well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Component:
  • Atf2 Microplate: 96 Wells (12 x 8 Well strips)
  • Atf2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Atf2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.316 ng/mL
• Specificity: Natural and recombinant Rat Cyclic AMP-dependent transcription factor ATF-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atf2
• Gene Full Name: activating transcription factor 2
• Alias Symbols: Activating transcription factor 2;cAMP response element-binding protein CRE-BP1;cAMP-dependent transcription factor ATF-2;cyclic AMP-dependent transcription factor ATF-2.
• NCBI Gene Id: 81647
• Protein Name: Q00969
• Description of Target: Cyclic AMP-dependent transcription factor ATF-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q00969
• Protein Accession #: NP_112280.1
• Nucleotide Accession #: NM_031018.1
• Protein Size (# AA): 487
• Molecular Weight: 52 kDa