ASB9 Antibody (OAGA00566)

Catalog No: OAGA00566

Size:100 ul

Sample(30 ug whole cell lysate)
A:HeLa S3
B:Hep G2
C:MOLT4
12% SDS PAGE
OAGA00566 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ASB9(OAGA00566) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ASB9(OAGA00566) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a member of the ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein family. Members of this family can interact with the elongin B-C adapter complex via their SOCS box domain and further complex with the cullin and ring box proteins to form E3 ubiquitin ligase complexes. They may function to mediate the substrate-recognition of the E3 ubiquitin ligases. A transcribed pseudogene of this gene has been identified on chromosome 15. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  Positive Controls: HeLaS3, HepG2, Molt-4
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 283 of ASB9 (Uniprot ID#Q96DX5)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ASB9
• Gene Full Name: Ankyrin repeat and SOCS box protein 9
• Alias Symbols: DKFZp564L0862 antibody, FLJ20636 antibody, MGC4954 antibody, ASB9 antibody, ankyrin repeat and SOCS box-containing 9 antibody, ASB-9 antibody, ankyrin repeat and suppressor of cytokine signaling box protein 9 antibody, ankyrin repeat and SOCS box protein
• NCBI Gene Id: 140462
• Protein Name: Ankyrin repeat and SOCS box protein 9
• Uniprot ID: Q96DX5
• Protein Accession #: NP_001026909.1
• Molecular Weight: 32 kDa