ARL2 Antibody - C-terminal region (OAAB15841)

Catalog No: OAAB15841

Size:400ul

Western blot analysis of ARL2 (arrow) using rabbit polyclonal ARL2 Antibody (D170) (Cat. # OAAB15841). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARL2 gene.

ARL2 Antibody (C-term) (Cat. # OAAB15841) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ARL2 Antibody (C-term) (Cat. # OAAB15841) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: ARL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca (2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle
  Cellular Location: Mitochondrion intermembrane space. Cytoplasm, cytoskeleton, centrosome. Nucleus. Cytoplasm. Note=The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to i
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ARL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the C-terminal region of human ARL2.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: ARL2
• Alias Symbols: ARFL2, MRCS1
• NCBI Gene Id: 402
• Protein Name: ADP-ribosylation factor-like protein 2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P36404
• Protein Accession #: NP_001186674.1
• Molecular Weight: 20878 Da