ARHGAP35 Antibody (OAAF00738)

Catalog No: OAAF00738 (Formerly GWB-ASC142)

Size:100 ug

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRF-1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human GRF-1.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: GRF-1 antibody detects endogenous levels of total GRF-1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Tikoo A., Gene 257:23-31(2000).
  Nagase T., DNA Res. 7:347-355(2000).
  Nakajima D., DNA Res. 9:99-106(2002)

Target Info

• Gene Symbol: ARHGAP35
• Gene Full Name: Rho GTPase activating protein 35
• Alias Symbols: glucocorticoid receptor DNA-binding factor 1;glucocorticoid receptor repression factor 1;GRF-1;GRLF1;P190A;P190-A;p190ARhoGAP;p190RhoGAP;rho GAP p190A;rho GTPase-activating protein 35.
• NCBI Gene Id: 2909
• Protein Name: Rho GTPase-activating protein 35
• Description of Target: Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621).
• Uniprot ID: Q9NRY4
• Protein Accession #: NP_004482.4
• Molecular Weight: 172 kDa