AR ELISA Kit (Human) (OKCD00824)

Catalog No: OKCD00824 (Formerly GWB-KBBLI7)

Size:96 Wells

Human AR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Androgen Receptor ELISA Kit (Human) (OKCD00824) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Androgen Receptor has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Androgen Receptor is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Androgen Receptorcaptured in well.
• ELISA Kit Range: 0.312-20 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Androgen Receptor were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Androgen Receptor were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-Androgen Receptor Microplate: 96 Wells (12 x 8 Well Strips)
  • Androgen Receptor Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Androgen Receptor Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Androgen Receptor (AR). No significant cross-reactivity or interference between Androgen Receptor (AR) and analogues was observed.
• Publications:

  High-Load Resistance Exercise Augments Androgen Receptor-DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content. Nutrients. 12, (2020). 33333818

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AR
• Gene Full Name: androgen receptor
• Alias Symbols: KD, AIS, AR8, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA
• NCBI Gene Id: 367
• Protein Name: androgen receptor
• Description of Target: #N/A
• Uniprot ID: P10275
• Protein Accession #: NP_000035
• Nucleotide Accession #: NM_000044.3
• Protein Size (# AA): 920