Catalog No: OKCD00824 (Formerly GWB-KBBLI7)
Size:96 Wells
Price: $650.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology Androgen Receptor ELISA Kit (Human) (OKCD00824) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Androgen Receptor has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Androgen Receptor is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Androgen Receptorcaptured in well.
ELISA Kit Range0.312-20 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Androgen Receptor were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Androgen Receptor were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-Androgen Receptor Microplate96 Wells (12 x 8 Well Strips)
Androgen Receptor Lyophilized Standard2 x 20 ng
100X Biotinylated Androgen Receptor Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Androgen Receptor (AR). No significant cross-reactivity or interference between Androgen Receptor (AR) and analogues was observed.

High-Load Resistance Exercise Augments Androgen Receptor-DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content. Nutrients. 12, (2020). 33333818

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAR
Gene Full Nameandrogen receptor
NCBI Gene Id367
Protein Nameandrogen receptor
Description of Target#N/A
Uniprot IDP10275
Protein Accession #NP_000035
Nucleotide Accession #NM_000044.3
Protein Size (# AA)920
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