Aposcreen Annexin V Apoptosis Kit : PE (OKSB00008)

Catalog No: OKSB00008

Size:100T

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-PE.

Product Info

• Host: Expressed in E. coli
• Conjugation: R-PE
• Application: FC
• Additional Information: Description: ApoScreen Annexin V Apoptosis Kit-PE employs a R-phycoerythrin-conjugated Annexin V (Annexin V-PE) in concert with 7-Aminoactinomycin D (7-AAD) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-PE. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, 7-AAD can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-PE and 7-AAD, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-PE or 7-AAD; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-PE only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm.
• Reconstitution and Storage: Store at 2-8C
• Application Kit: - 1 mL Annexin V- FITC: Store at 2- 8 C. Use as directed; no pre- dilution necessary
  - 1 mL propidium iodide: Store at 2- 8 C. Use as directed; no pre- dilution necessary. Avoid skin contact and dispose ofproperly
  - 7 x 1.5 mL 10X binding buffer: Store at 2- 8 C. Dilute 1 part 10X buffer with 9 parts dH20 prior to use
  Note: Annexin V is produced by Aviva Systems Biology
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• Assay Info: The ApoScreen Annexin V Apoptosis Kit employs a fluorescein- labeled annexin V (annexin V- FITC) in concert withpropidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis,cells begin to display PS on the outer cell membrane where it is readily detectable by staining the cells with annexin VFITC.As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readilymove across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lostmembrane integrity through mechanisms including necrosis. When cells are double stained with annexin V- FITC and PI,three different cell populations may be observed (Figure 2): (i) live cells that do not stain with either annexin V- FITC or PI;(ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with annexin V- FITC only. Analysis canbe performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells canbe examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.