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APOB100 ELISA Kit (Rabbit) (OKWB00400)

96 Wells
$400.00
In Stock
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Predicted Species Reactivity:
Rabbit
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
APOB100
Protein Name:
Apolipoprotein B-100
Swissprot Id:
Q7M2U9
Sample Type:
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity:
46.9 ng/mL
Kit Range:
0.078 - 5 ug/mL
Kit Reproducibility:
Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Apob100 ELISA Kit (Rabbit) (OKWB00400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apob100 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apob100 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apob100 captured in the well.
Kit Component:
ComponentAmount
Apob100 Microplate96 Wells (12 x 8 Well strips)
Apob100 Lyophilized Standard2 x 5 ug
100X Apob100 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Linearity:
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-98%89-104%87-103%85-92%
EDTA Plasma (n=5)83-97%90-100%83-101%82-85%
Heparin Plasma (n=5)84-99%81-99%81-91%80-96%
Kit Recovery:
Sample TypeRangeMean
Serum (n=5)85-10091
EDTA Plasma (n=5)95-10498
Heparin Plasma (n=5)87-9590
Kit Detection Method:
Colorimetric, OD 450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Type: Quantitative Sandwich ELISA

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