AP2A2 Antibody - C-terminal region (ARP59974_P050)

Catalog No: ARP59974_P050

WB Suggested Anti-AP2A2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Lung

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AP2A2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 79%; Guinea Pig: 79%; Human: 100%; Mouse: 79%; Rat: 79%
• Peptide Sequence: Synthetic peptide located within the following region: LAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADL
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-AP2A2 (ARP59974_P050) antibody is Catalog # AAP59974 (Previous Catalog # AAPP46132)
• Subunit: alpha-2

Target Info

• Gene Symbol: AP2A2
• Gene Full Name: Adaptor-related protein complex 2, alpha 2 subunit
• Alias Symbols: HIP9, HYPJ, ADTAB, HIP-9, CLAPA2
• NCBI Gene Id: 161
• Protein Name: AP-2 complex subunit alpha-2
• Description of Target: AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif .
• Uniprot ID: O94973
• Protein Accession #: NP_036437
• Nucleotide Accession #: NM_012305
• Protein Size (# AA): 939
• Molecular Weight: 104kDa
• Protein Interactions: PIP5K1C; UBC; ATP1A1; SUMO2; EGFR; RPA3; RPA2; RPA1; BIN1; PPP6R3; PAN2; SMAD1; FN1; DAB2; VCP; APP; AP2A1; SHC1; GRK5; EPS15; AP2M1; CLTC; GRB2; ARRB2; Rnf111; TERF2; DNAJC6; SNAP91; EPN1; AP2A2; NUMB; AP1S1; DNM1; AP2B1; AMPH; HTT; ATR; ATM; RALBP1;