ANXA5 Recombinant Protein (Human) (OPED00007)

Catalog No: OPED00007

Size:100 tests

Figure A: FACS histogram for the fluorescence intensity measured in FL-1 (FITC) channel. Jurkat cells were incubated for 2 or 6 hours with medium (untreated) or with 100ng/ml rhsFasL in the presence of 1ug/ml Enhancer for Ligands (rhsFasL Se) Figure B: Comparison of ALEXIS and Competitor Annexin-V. Jurkat cells were incubated for 2 or 6 hours with medium (background) or with 100ng/ml rhs FasL in the presence of 1ug/ml Enhancer for Ligands (rhs FasL Set,). Cells were stained with Annexin-V-FITC for 10 min. and then analyzed on FACS. Shown here is the increase in Annexin-V-FITC positive cells as increase from the background. Same amounts of Annexin-V-FITC were compared. Method: Jurkat cells were incubated for 2 or 6 hours with medium (background) or with 100ng/ml rhsFasL in the presence of 1ug/ml Enhancer for Ligands. 2x105 cells per staining were washed once in PBS, resuspended in binding buffer and stained with 5ul Annexin-V-FITC for 10 min. at RT in the dark. Cells were then washed once, resuspended in binding buffer, and analyzed by FACS.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
• Conjugation: FITC
• Application: FC
• Reconstitution and Storage: Store at 4C
• Purification: Affinity purified.
• Purity: >90% (SDS-PAGE)
• Application Info: Detection of apoptotic cells by flow cytometry.

Target Info

• Gene Symbol: ANXA5
• Gene Full Name: annexin A5
• Alias Symbols: anchorin CII;annexin A5;annexin V;annexin-5;ANX5;calphobindin I;CBP-I;endonexin II;ENX2;epididymis secretory protein Li 7;HEL-S-7;lipocortin V;PAP-I;placental anticoagulant protein 4;placental anticoagulant protein I;PP4;RPRGL3;thromboplastin inhibitor;VAC-alpha;vascular anticoagulant-alpha.
• NCBI Gene Id: 308
• Protein Name: Annexin A5
• Description of Target: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
• Uniprot ID: P08758
• Protein Accession #: NP_004337.2
• Nucleotide Accession #: NM_004346.3
• Protein Size (# AA): Recombinant
• Molecular Weight: 52 kDa