ANXA1 ELISA Kit (Chicken) (OKEH07368)

Catalog No: OKEH07368

Size:96T

Chicken ANXA1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ANXA1 ELISA Kit (Chicken) (OKEH07368) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANXA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANXA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANXA1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.9% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • ANXA1 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA1 Lyophilized Standard: 2
  • 100X Biotinylated ANXA1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing (By similarity). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (By similarity). Has no effect on unstimulated T-cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton.
  Subcellular Location: Nucleus Cytoplasm Cell projection Cilium Basolateral cell membrane Lateral cell membrane Cell membrane Peripheral membrane protein Apical cell membrane Membrane Peripheral membrane protein Early endosome Cytoplasmic vesicle membrane Peripheral membrane protein Endosome membrane Peripheral membrane protein Secreted Secreted Extracellular space Cell membrane Peripheral membrane protein Extracellular side Secreted Exosome Cytoplasmic vesicle Secretory vesicle lumen Cell projection Phagocytic cup Colocalizes with actin fibers at phagocytic cups. Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles. Secretion is increased in response to wounding and inflammation (By similarity). Detected in gelatinase granules in resting neutrophils. Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect. Displays calcium-dependent binding to phospholipid membranes (By similarity).
  Unigene: Gga.4884
  SMR: Q92108
  String: 9031.ENSGALP00000032184
  Pfam: PF00191
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.087ng/mL
• Specificity: Natural and recombinant Chicken Annexin A1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANXA1
• Alias Symbols: Annexin I;Annexin-1;Calpactin II;Calpactin-2;Chromobindin-9;Lipocortin I;p35;Phospholipase A2 inhibitory protein.
• Protein Name: Q92108
• Description of Target: Annexin A1
• Uniprot ID: https://www.uniprot.org/uniprot/Q92108