Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)

Catalog No: OAAI00438

Size:100UG

Western Blot: The Cell lysate derived from Hela (lane 2) and HT-29 (Lane 3) were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immuno- blotted by Rb anti Villin 2 (Cat#OAAI00438) at 1:500. An immunoreactive band around ~80 kDa was observed.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Villin 2, also known as Ezrin, p81, 80K, or cytovillin, is a cytoplasmic peripheral membrane protein which is enriched in microvilli and other cell surface structures. Villin belongs to a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Villin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers.
  Positive Control: Hela cells
  Cellular Location: Cytoplasmic/Peripheral membrane
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the C-term portion of human Ezrin protein. This sequence is identical to human, rat, mouse, dog, Monkey, zebra fish, and human origins.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: EZR
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Krieg,J. and Hunter,T. Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein Ezrin. J. Biol. Chem. 267 (27), 19258-19265 (1992).
  

Target Info

• Gene Symbol: EZR
• Gene Full Name: ezrin
• Alias Symbols: CVIL;CVL;cytovillin 2;epididymis secretory protein Li 105;ezrin;HEL-S-105;p81;VIL2;villin 2 (ezrin).
• NCBI Gene Id: 7430
• Description of Target: Villin 2, also known as Ezrin, p81, 80K, or cytovillin, is a cytoplasmic peripheral membrane protein which is enriched in microvilli and other cell surface structures, Villin belongs to a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Villin serves as a tyrosine kinase substRate, and is phosphorylated in EGF-stimulated cells. This protein plays a key role in cell surface structure adhesion, migRation and organization, and it has been implicated in various Human cancers.
• Uniprot ID: P15311
• Molecular Weight: 92.5