Anti-LRP6 Phospho-specific Antibody (OAAI00462)

Catalog No: OAAI00462

Size:100UG

Western Blot: The cell lysate derived from conditioned 3T3 was immunoprobed by Rabbit anti-LRP6(pT1479) (Cat#OAAI00462) at 1:500. An immunoreactive band is observed around ~180kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).

Dot Blot: 1 ?g peptide was blot onto NC membrane (A- Non-related phosphospecific peptide; B-LRP6(pT1479); C: Non- Phosphopeptide LRP6) was blotted by Rabbit anti-LRP6(pT1479) (Cat#OAAI00462) a 1:2000 dilution.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Low-desnsity lipoprotein receptor (LDLR)-related protein 6 (LRP6) is a member of the LDLR family which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LRP5 cuases several human diseases including bone mineral density variability1, endosteal hyperostosis, autosomal dominant osteopetrosis type I etc. The V171 mutation in LRP5 causes high bone density by impairing the action of a normal antagonist of the Wnt pathway and thus increasing Wnt signaling. The activity of LRP5 and LRP6 can be inhibited by binding of some members of Dickkopf (DKK) family proteins. Upon stimulation with Wnt, LRP5 and LRP6 are phosphorylated at multiple sites including Thr 1479, Ser 1490 and Thr 1492 by kinases such as GSK-3 and CK1.
  Positive Control: Human breast cancer
  Cellular Location: Cytoplasm, Nucleus
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the epitope KGTYF at a phosphorylation threonine1479 in LRP6 protein
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: LRP6
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Zahid Khan et al. (2007), Molecular and Cellular Biology, 27(20), 7291-7301.

Target Info

• Gene Symbol: LRP6
• Gene Full Name: LDL receptor related protein 6
• Alias Symbols: ADCAD2;low-density lipoprotein receptor-related protein 6;LRP-6;STHAG7.
• NCBI Gene Id: 4040
• Description of Target: Low-desnsity lipoprotein receptor (LDLR)-related protein 6 (LRP6) is a member of the LDLR family which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LRP5 cuases several Human diseases including bone mineral density variability1, endosteal hyperostosis, autosomal dominant osteopetrosis type I etc. The V171 mutation in LRP5 causes high bone density by impairing the action of a normal antagonist of the Wnt pathway and thus increasing Wnt signaling. The activity of LRP5 and LRP6 can be inhibited by binding of some members of Dickkopf (DKK) family proteins. Upon stimulation with Wnt, LRP5 and LRP6 are phosphorylated at multiple sites including Thr 1479, Ser 1490 and Thr 1492 by kinases such as GSK-3 and CK1.
• Uniprot ID: O75581
• Molecular Weight: 180