Anti-GABA Alpha Receptor, alpha 3 -Subunit (OAPC00121)

Catalog No: OAPC00121

Size:100 ul

Western blot of mouse brain lysates from wild type (Control) and alpha 3-knockout (alpha K/O) animals showing specific immunolabeling of the ~51k alpha 3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 3-knockout animals.

Immunostaining of rat amygdala showing labeling of GABAA alpha 3 subunit.

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid 10mM HEPES (pH 7.5), 150mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC, DB
• Reconstitution and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
• Purification: Affinity purified
• Concentration: Varies by lot. See vial for concentration.
• Specificity: Specific for the ~51k alpha 3-subunit of the GABAA-R receptor in Western blots of rat and mouse brain lysate.
• Application Info: DB: 1:1000
  WB: 1:1000
  IHC: 1:100
• Reference: McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha 1-subtype. Nature Neurosci 3:587-592.
  Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha 1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
  Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma 2-subunit. Biochem Pharmacol 68:1621-1629.
  Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
  Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAAreceptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.
  Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR,
  Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding
  GABAA receptor gene family. Ann NY Acad Sci 868:645-653.
• Quality Control: Dot blots and Western blots performed with each lot.

Target Info

• Gene Symbol: GABRA3
• NCBI Gene Id: 24947
• Protein Name: Gamma-aminobutyric acid receptor subunit alpha-3
• Description of Target: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
• Uniprot ID: P20236
• Molecular Weight: ~51kDa