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Catalog No: OKCD02363
Size:96WELLS
Price: $700.00
SKU
OKCD02363
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Datasheets/ManualsPrintable datasheet for ANGPT1 ELISA Kit (Dog) (OKCD02363)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%95-102%78-98%82-101%
EDTA Plasma (n=5)83-97%99-105%88-95%79-92%
Heparin Plasma (n=5)93-101%78-101%79-92%79-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 1 (ANGPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin 1 (ANGPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin 1 (ANGPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiopoietin 1 (ANGPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-10597
EDTA Plasma (n=5)83-10190
Heparin Plasma(n=5)91-10195
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ANGPT1 Microplate96 Wells (12 x 8 Well strips)
ANGPT1 Lyophilized Standard2
100X Biotinylated ANGPT1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.93 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiopoietin 1 (ANGPT1). No significant cross-reactivity or interference between Angiopoietin 1 (ANGPT1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolANGPT1
Gene Full Nameangiopoietin 1
Alias SymbolsANG1;ANG-1;angiopoietin-1.
NCBI Gene Id403656
Protein NameAngiopoietin-1
Description of TargetBinds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development (By similarity).
Uniprot IDQ60FC1
Protein Accession #NP_001005754.1
Nucleotide Accession #NM_001005754.2
Protein Size (# AA)497
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