ANAPC2 Antibody - C-terminal region (OAAB19015)

Catalog No: OAAB19015

Size:400ul

Western blot analysis of lysate from human testis tissue lysate, using ANAPC2 Antibody (C-term) (Cat # OAAB19015). OAAB19015 was diluted at 1: 1000. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody. Lysate at 20ug.

Immunohistochemical analysis of paraffin-embedded H. thymus section using ANAPC2 Antibody (C-term) (Cat # OAAB19015). OAAB19015 was diluted at 1: 25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ANAPC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of human ANAPC2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Application Info: IF: 1:25
  IHC-P: 1:25
  WB: 1:1000
• Reference: Yu H.,et al.Science 279:1219-1222(1998).
  Humphray S.J.,et al.Nature 429:369-374(2004).
  Nagase T.,et al.DNA Res. 7:65-73(2000).
  Nakajima D.,et al.DNA Res. 9:99-106(2002).
  Tang Z.,et al.Mol. Biol. Cell 12:3839-3851(2001).
  

Target Info

• Gene Symbol: ANAPC2
• Alias Symbols: APC2
• NCBI Gene Id: 29882
• Description of Target: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.
• Uniprot ID: Q9UJX6; Q8BZQ7
• Molecular Weight: 94 kDa