AMY1A ELISA Kit (Human) (OKCD07429)

Catalog No: OKCD07429

Size:96WELLS

AMY1A ELISA Kit (Human) (OKCD07429) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Salivary Alpha Amylase (AMY1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Salivary Alpha Amylase (AMY1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Salivary Alpha Amylase (AMY1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Salivary Alpha Amylase (AMY1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AMY1A Microplate: 96 Wells (12 x 8 Well strips)
  • AMY1A Lyophilized Standard: 2
  • 100X AMY1A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, saliva and other biological fluids
• Sensitivity: < 1.22ng/mL

Target Info

• Gene Symbol: AMY1A|AMY1B|AMY1C
• Gene Full Name: amylase alpha 1A|amylase alpha 1B|amylase alpha 1C
• Alias Symbols: 1,4-alpha-D-glucan glucanohydrolase 1;alpha-amylase 1;alpha-amylase 1A;alpha-amylase 1B;alpha-amylase 1C;AMY1;amylase alpha 1A (salivary);amylase alpha 1B (salivary);amylase alpha 1C (salivary);amylase, salivary, alpha-1A;amylase, salivary, alpha-1B;amylase, salivary, alpha-1C;glycogenase;salivary alpha-amylase;salivary amylase alpha 1A;salivary amylase alpha 1B;salivary amylase alpha 1C.
• NCBI Gene Id: 276|277|278
• Protein Name: Alpha-amylase 1|Alpha-amylase 1A|Alpha-amylase 1B|Alpha-amylase 1C
• Description of Target: Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
• Uniprot ID: P04745
• Protein Accession #: NP_001008219.1
• Nucleotide Accession #: NM_001008218.1