AMY1 ELISA Kit (Rat) (OKCD04303)

Catalog No: OKCD04303

Size:96WELLS

Rat AMY1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-103%	83-93%	92-99%	94-105%
  EDTA Plasma (n=5)	96-104%	89-103%	79-104%	98-105%
  Heparin Plasma (n=5)	87-94%	82-96%	85-96%	79-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Salivary Alpha Amylase (AMY1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Salivary Alpha Amylase (AMY1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Salivary Alpha Amylase (AMY1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Salivary Alpha Amylase (AMY1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	81-98	88
  EDTA Plasma (n=5)	82-89	85
  Heparin Plasma (n=5)	93-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salivary Alpha Amylase (AMY1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Amy1 Microplate: 96 Wells (12 x 8 Well strips)
  • Amy1 Lyophilized Standard: 2
  • 100X Amy1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: 0.28 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Amy1a
• Gene Full Name: amylase, alpha 1A
• Alias Symbols: alpha-amylase 1;Amy1;amylase 1, salivary;amylase, alpha 1A (salivary).
• NCBI Gene Id: 24203
• Protein Name: Alpha-amylase
• Uniprot ID: Q5I0L0