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Catalog No: OKEH00967
Size:96T
Price: $525.00
SKU
OKEH00967
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ALOX5 ELISA Kit (Human) (OKEH00967) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALOX5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALOX5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALOX5 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
ALOX5 Microplate96 Wells (12 x 8 Well strips)
ALOX5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ALOX5 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Arachidonate 5-lipoxygenase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolALOX5
Gene Full Namearachidonate 5-lipoxygenase
Alias Symbols5-LO;5-LOX;5LPG;arachidonic acid 5-lipoxygenase;leukotriene A4 synthase;LOG5;LOX-5;polyunsaturated fatty acid 5-lipoxygenase.
NCBI Gene Id240
Protein NameP09917
Description of TargetArachidonate 5-lipoxygenase
Uniprot IDhttps://www.uniprot.org/uniprot/P09917
Protein Accession #NP_000689.1
Nucleotide Accession #NM_000698.3
Protein Size (# AA)674
Molecular Weight77 kDa
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