ALDH3A1 ELISA Kit (Human) (OKEH07723)

Catalog No: OKEH07723

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ALDH3A1 ELISA Kit (Human) (OKEH07723) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDH3A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALDH3A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDH3A1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.8% (n = 20)
  Mean Inter-assay CV%: <=10.4% (n = 20)
• ELISA Kit Component:
  • ALDH3A1 Microplate: 96 Wells (12 x 8 Well strips)
  • ALDH3A1 Lyophilized Standard: 2
  • 100X Biotinylated ALDH3A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light.
  Subcellular Location: Cytoplasm
  Unigene: Hs.531682
  SMR: P30838
  String: 9606.ENSP00000225740
  KEGG: hsa:218
  MIM: 100660
  Pfam: PF00171
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Human Aldehyde dehydrogenase, dimeric NADP-preferring
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ALDH3A1
• Gene Full Name: aldehyde dehydrogenase 3 family member A1
• Alias Symbols: Aldehyde dehydrogenase 3;Aldehyde dehydrogenase family 3 member A1;aldehyde dehydrogenase isozyme 3;aldehyde dehydrogenase type III;aldehyde dehydrogenase, dimeric NADP-preferring;ALDH3;ALDHIII;stomach aldehyde dehydrogenase.
• NCBI Gene Id: 218
• Protein Name: P30838
• Description of Target: Aldehyde dehydrogenase, dimeric NADP-preferring
• Uniprot ID: https://www.uniprot.org/uniprot/P30838
• Protein Accession #: NP_000682.3
• Nucleotide Accession #: NM_000691.4