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Datasheets/Manuals | Printable datasheet for AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537) |
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Predicted Species Reactivity | Bovine|Chicken|Dog|Human|Mouse|Porcine|Rabbit|Rat|Sheep |
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Product Format | Liquid. Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Subcellular location: Cytoplasm, Nucleus |
:: | Modification: Phosphorylation (Ser472) |
Reconstitution and Storage | -20°C |
Immunogen | KLH conjugated synthetic phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472 |
Purification | Protein A Purified |
Concentration | 1 ug/ul |
Specificity | This antibody will cross react with Akt1 when phosphorylated at Ser473 and Akt2 when phosphorylated at Ser474. |
Application Info | WB: 1:100 - 1000 IHC-P: : 1:100 - 500 |
Gene Symbol | AKT3 |
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Gene Full Name | AKT serine/threonine kinase 3 |
Alias Symbols | MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PRKBG;Protein kinase Akt-3;Protein kinase B gamma;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma). |
NCBI Gene Id | 10000 |
Protein Name | RAC-gamma serine/threonine-protein kinase |
Description of Target | AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
Uniprot ID | Q9Y243 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Chicken|Dog|Human|Mouse|Porcine|Rabbit|Rat|Sheep".
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How long will it take to receive "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
This item is available "3 to 5 business days.".
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What buffer format is "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)" provided in?
This item is provided in "Liquid. Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
This target may also be called "MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PRKBG;Protein kinase Akt-3;Protein kinase B gamma;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)." in publications.
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What is the shipping cost for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AKT3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AKT3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AKT3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AKT3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AKT3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AKT3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.