AKT1/2/3 Antibody (Phospho-Ser472/Ser473/Ser474) (OABF01537)

Catalog No: OABF01537

Size:100UL

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-phospho-AKT3 (Ser472) Polyclonal Antibody, Unconjugated (OABF01537) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-AKT3 (Ser472) Polyclonal Antibody, Unconjugated (OABF01537) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37C and DAPI staining

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Dog|Human|Mouse|Porcine|Rabbit|Rat|Sheep
• Product Format: Liquid. Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Subcellular location: Cytoplasm, Nucleus
  Modification: Phosphorylation (Ser472)
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Specificity: This antibody will cross react with Akt1 when phosphorylated at Ser473 and Akt2 when phosphorylated at Ser474.
• Application Info: WB: 1:100 - 1000
  IHC-P: : 1:100 - 500

Target Info

• Gene Symbol: AKT3
• Gene Full Name: AKT serine/threonine kinase 3
• Alias Symbols: MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PRKBG;Protein kinase Akt-3;Protein kinase B gamma;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma).
• NCBI Gene Id: 10000
• Protein Name: RAC-gamma serine/threonine-protein kinase
• Description of Target: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
• Uniprot ID: Q9Y243