AGL ELISA kit (Human)

Catalog No: OKCD09589

Size:96WELLS

Human AGL ELISA standard curve.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Debranching Enzyme (AGL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Debranching Enzyme (AGL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Debranching Enzyme (AGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycogen Debranching Enzyme (AGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	94-105	99
  EDTA plasma (n=5)	81-97	92
  Heparin plasma (n=5)	79-93	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycogen Debranching Enzyme (AGL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycogen Debranching Enzyme (AGL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-AGL Microplate: 96 Wells (12 x 8 Well Strips)
  • AGL Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated AGL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycogen Debranching Enzyme (AGL). No significant cross-reactivity or interference between Glycogen Debranching Enzyme (AGL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AGL
• Gene Full Name: amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
• Alias Symbols: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase;amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided;GDE;glycogen debrancher;glycogen debranching enzyme;glycogen debranching protein.
• NCBI Gene Id: 178
• Protein Name: Glycogen debranching enzyme
• Description of Target: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
• Uniprot ID: P35573
• Protein Accession #: NP_000019.2
• Nucleotide Accession #: NM_000028.2
• Protein Size (# AA): 1532