Adenovirus Type 40 Hexon Protein (OONB00082)

Catalog No: OONB00082

Product Info

• Product Format: Liquid. 10mM Bis Tris Propane, pH 7.2 ,375 mM NaCl, 0.09% Sodium Azide
• Reconstitution and Storage: Ships at ambient temperature. Aliquot and freeze at -20°C , recommended at -80°C. Avoid multiple freeze/thaw cycles.
• Source: Native
• Concentration: Adjusted to 0.291 mg/ml by BCA Assay, using BSA standards in 10mM Bis Tris propane pH 7.2
• Purity: > 95% pure by SDS-PAGE
• Description: Purified native hexon protein from Adenovirus 5. Hexon is the major Adenovirus capsid protein. It is present as 240 copies which form 20 facets of the icosahedral capsid.

Target Info

• Description of Target: Hexon protein naturally self assembles as part of the virus life cycle. When the protein is purified we also see evidence of self assembly such as rapid loss of protein upon serial dilution and precipitation. The storage buffer and ELISA coating buffer play an important role in the likelihood of self assembly and thereby can affect results when using purified hexon as an antigen in ELISA and other assays. Many common ELISA coating buffers increase self assembly in hexon protein as they are designed to increase efficiency of antigen adsorption. This results in a very high signal with the initial dilution but then sensitivity tails off very rapidly. Diluting and coating the hexon in water alone slightly increases sensitivity but reduces strength; coating and diluting in the presence of a small amount of stabilizing protein (such as BSA) seems to increase sensitivity for antigen coating significantly (although too much BSA can block high concentration of hexon).