ADAMTS4 ELISA Kit (Bovine) (OKEH08427)

Catalog No: OKEH08427

Size:96T

Bovine ADAMTS4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ADAMTS4 ELISA Kit (Bovine) (OKEH08427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTS4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMTS4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAMTS4 captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.4% (n = 20)
  Mean Inter-assay CV%: <=10.8% (n = 20)
• ELISA Kit Component:
  • ADAMTS4 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAMTS4 Lyophilized Standard: 2
  • 100X Biotinylated ADAMTS4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.
  Subcellular Location: Secreted Extracellular space Extracellular matrix
  Unigene: Bt.15675
  SMR: Q9TT93
  String: 9913.ENSBTAP00000017583
  KEGG: bta:286806
  Pfam: PF05986
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine A disintegrin and metalloproteinase with thrombospondin motifs 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADAMTS4
• Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif 4
• Alias Symbols: A disintegrin and metalloproteinase with thrombospondin motifs 4;a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4;ADAM-TS 4;ADAMTS-4;ADAM-TS4;ADMP-1;aggrecanase-1.
• NCBI Gene Id: 286806
• Protein Name: Q9TT93
• Description of Target: A disintegrin and metalloproteinase with thrombospondin motifs 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q9TT93
• Protein Accession #: NP_858053.1
• Nucleotide Accession #: NM_181667.1