ACO2 ELISA Kit (Pig) (OKEH07867)

Catalog No: OKEH07867

Size:96T

Pig ACO2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	104-114% (n = 20)	105-117% (n = 20)	105-114% (n = 20)	101-111% (n = 20)
  EDTA plasma(n=5)	109-119% (n = 20)	102-112% (n = 20)	109-120% (n = 20)	108-117% (n = 20)
  heparin plasma(n=5)	84-94% (n = 20)	114-124% (n = 20)	90-98% (n = 20)	104-115% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ACO2 ELISA Kit (Pig) (OKEH07867) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACO2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACO2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACO2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.8% (n = 20)
  Mean Inter-assay CV%: <=11.3% (n = 20)
• ELISA Kit Component:
  • ACO2 Microplate: 96 Wells (12 x 8 Well strips)
  • ACO2 Lyophilized Standard: 2
  • 100X Biotinylated ACO2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  Subcellular Location: Mitochondrion
  Unigene: Ssc.4263
  SMR: P16276
  String: 9823.ENSSSCP00000000070
  KEGG: ssc:396999
  Pfam: PF00330
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.068ng/mL
• Specificity: Natural and recombinant Pig Aconitate hydratase, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACO2
• Gene Full Name: aconitase 2
• Alias Symbols: aconitate hydratase, mitochondrial;citrate hydro-lyase;heart aconitase.
• NCBI Gene Id: 396999
• Protein Name: P16276
• Description of Target: Aconitate hydratase, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/P16276
• Protein Accession #: NP_999119.1
• Nucleotide Accession #: NM_213954.1