ACAN ELISA Kit (Bovine) (OKEH08445)

Catalog No: OKEH08445

Size:96T

Bovine ACAN ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ACAN ELISA Kit (Bovine) (OKEH08445) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACAN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACAN captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.5% (n = 20)
  Mean Inter-assay CV%: <=8.1% (n = 20)
• ELISA Kit Component:
  • ACAN Microplate: 96 Wells (12 x 8 Well strips)
  • ACAN Lyophilized Standard: 2
  • 100X Biotinylated ACAN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
  Subcellular Location: Secreted Extracellular space Extracellular matrix
  Unigene: Bt.4953
  String: 9913.ENSBTAP00000021512
  KEGG: bta:280985
  Pfam: PF00008
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.098ng/mL
• Specificity: Natural and recombinant Bovine Aggrecan core protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACAN
• Gene Full Name: aggrecan
• Alias Symbols: AGC1;aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122);aggrecan core protein;aggrecan epidermal growth factor-like domain-1;cartilage-specific proteoglycan core protein;CSPCP.
• NCBI Gene Id: 280985
• Protein Name: P13608
• Description of Target: Aggrecan core protein
• Uniprot ID: https://www.uniprot.org/uniprot/P13608
• Protein Accession #: NP_776406.1
• Nucleotide Accession #: NM_173981.2