ABCG1 ELISA Kit (Human) (OKEH08736)

Catalog No: OKEH08736

Size:96T

Human ABCG1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	102-113% (n = 20)	84-93% (n = 20)	103-112% (n = 20)	83-92% (n = 20)
  EDTA plasma(n=5)	105-115% (n = 20)	106-115% (n = 20)	110-120% (n = 20)	97-107% (n = 20)
  heparin plasma(n=5)	103-113% (n = 20)	90-100% (n = 20)	108-119% (n = 20)	103-116% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ABCG1 ELISA Kit (Human) (OKEH08736) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCG1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.3% (n = 20)
  Mean Inter-assay CV%: <=9.0% (n = 20)
• ELISA Kit Component:
  • ABCG1 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCG1 Lyophilized Standard: 2
  • 100X Biotinylated ABCG1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
  Subcellular Location: Endoplasmic reticulum membrane Multi-pass membrane protein Golgi apparatus membrane Multi-pass membrane protein Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
  Unigene: Hs.124649
  SMR: P45844
  String: 9606.ENSP00000354995
  KEGG: hsa:9619
  MIM: 603076
  Pfam: PF01061
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.075ng/mL
• Specificity: Natural and recombinant Human ATP-binding cassette sub-family G member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABCG1
• Gene Full Name: ATP binding cassette subfamily G member 1
• Alias Symbols: ABC transporter 8;ABC8;ATP-binding cassette sub-family G member 1;ATP-binding cassette transporter 8;ATP-binding cassette transporter member 1 of subfamily G;ATP-binding cassette, sub-family G (WHITE), member 1;homolog of Drosophila white;White protein homolog;white protein homolog (ATP-binding cassette transporter 8);WHITE1.
• NCBI Gene Id: 9619
• Protein Name: P45844
• Description of Target: ATP-binding cassette sub-family G member 1
• Uniprot ID: https://www.uniprot.org/uniprot/P45844
• Protein Accession #: NP_004906.3
• Nucleotide Accession #: NM_004915.3