8ISOPG ELISA Kit (Human) (OKEH00590)

Catalog No: OKEH00590

Size:96T

Human 8ISOPG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology 8ISOPG ELISA Kit (Human) (OKEH00590) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human 8ISOPG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 8ISOPG and incubated. The 8ISOPG found in the sample or standards competes with the biotinylated 8ISOPG for limited binding sites on the immobilized anti-human 8ISOPG antibody. Excess unbound biotinylated 8ISOPG and sample or standard 8ISOPG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 8ISOPG captured in the well and inversely proportional to the amount of 8ISOPG which was contained in the sample or standard.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 10.2% (n = 20)
• ELISA Kit Component:
  • Anti-8ISOPG Microplate: 96 Wells (12 x 8 Well strips)
  • 8ISOPG Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X 8ISOPG-Biotin Complex: 1 x 60 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 10 pg/mL
• Specificity: Natural and recombinant Human 8-iso-Prostaglandin F2α
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: 8ISOPG
• Gene Full Name: 8-iso-prostaglandin
• Alias Symbols: 8-iso-prostaglandin
• Protein Name: 8ISOPG