Size:96 Wells
$469.00
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SKU
OKEH00590
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8ISOPG ELISA Kit (Human) (OKEH00590)

Catalog#: OKEH00590
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman
ApplicationELISA
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology 8ISOPG ELISA Kit (Human) (OKEH00590) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human 8ISOPG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 8ISOPG and incubated. The 8ISOPG found in the sample or standards competes with the biotinylated 8ISOPG for limited binding sites on the immobilized anti-human 8ISOPG antibody. Excess unbound biotinylated 8ISOPG and sample or standard 8ISOPG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 8ISOPG captured in the well and inversely proportional to the amount of 8ISOPG which was contained in the sample or standard.
Kit Range15.6 - 1000pg/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
Kit Component
ComponentAmount
Anti-8ISOPG Microplate96 Wells (12 x 8 Well strips)
8ISOPG Lyophilized Standard2
Sample Diluent1 x 20 mL
100X 8ISOPG-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Human 8-iso-Prostaglandin F2α
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene Symbol8ISOPG
Official Gene Full Name8-iso-prostaglandin
Alias Symbols8-iso-prostaglandin
Protein Name8ISOPG
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