5-Methyltetrahydrofolate ELISA Kit (OKCD02251)

Catalog No: OKCD02251 (Formerly GWB-GVPMN5)

Size:96WELLS

5-Me-THF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-96%	80-95%	98-105%	93-102%
  EDTA plasma(n=5)	81-102%	91-104%	85-92%	96-103%
  heparin plasma(n=5)	99-105%	93-101%	96-105%	84-95%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Me-THF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Me-THF analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Me-THF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Me-THF in the sample.
• ELISA Kit Range: 1.11-90ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	84-99	92
  EDTA plasma (n=5)	96-105	102
  Heparin Plasma (n=5)	80-102	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Methyltetrahydrofolate (5-Me-THF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Methyltetrahydrofolate (5-Me-THF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-5-Methyltetrahydrofolate Microplate: 96 Wells (12 x 8 Well Strips)
  • 5-Methyltetrahydrofolate Lyophilized Standard: 2
  • 100X 5-Methyltetrahydrofolate Biotin Complex: 120 uL
  • 100X Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Biotin Complex Diluent: 1 x 12 mL
  • Avidin/HRP Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Additional Information: CAS: 134-35-0
  PubChem: 444412
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.39 ng/mL
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: 5-ME-THF
• Alias Symbols: Levomefolic acid; 31690-09-2; LMSR; 5-Methyltetrahydrofolate; Levomefolinic acid
• Protein Name: 5-Methyltetrahydrofolate
• Description of Target: 5-Methyltetrahydrofolate is a methylated derivate of tetrahydrofolate. It is generated by methylenetetrahydrofolate reductase from 5,10-methylenetetrahydrofolate and used to recycle homocysteine back to methionine by 5-methyltetrahydrofolate-homocysteine methyltransferases (also called methionine synthases).