25-OH Vitamin D ELISA Kit (OKEH02569)

Catalog No: OKEH02569

Size:96 Wells

25-OH Vitamin D ELISA Kit (OKEH02569) standard curve.

Product Info

• Predicted Species Reactivity: All
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology 25-OH Vitamin D ELISA Kit (OKEH02569) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-25-OH Vitamin D antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 25-OH Vitamin D and incubated. The 25-OH Vitamin D found in the sample or standards competes with the biotinylated 25-OH Vitamin D for limited binding sites on the immobilized anti-25-OH Vitamin D antibody. Excess unbound biotinylated 25-OH Vitamin D and sample or standard 25-OH Vitamin D is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 25-OH Vitamin D captured in the well and inversely proportional to the amount of 25-OH Vitamin D which was contained in the sample or standard.
• ELISA Kit Range: 3.12 - 200nmol/L
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.1% (n = 20)
• ELISA Kit Component:
  • Anti-25-OH Vitamin D Microplate: 96 Wells (12 x 8 Well strips)
  • 25-OH Vitamin D Lyophilized Standard: 2 x 200 nmol
  • Sample Diluent: 1 x 20 mL
  • 100X 25-OH Vitamin D-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: 25OHVD
  Target Name: 25-OH Vitamin D
  Pubchem: 5283731
  Chemical formula: C27H44O2
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 1.27nmol/L
• Specificity: Natural and recombinant General 25-OH Vitamin D
• Publications:

  Anaparti, V. et al. Circulating levels of free 25(OH)D increase at the onset of rheumatoid arthritis. PLoS One. 2019 14(9): e0219109. 31557191

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: 25OHVD, 25-OH Vitamin D, 25OHVD, Calcifediol, Calcidiol
• Protein Name: 25-OH Vitamin D
• Description of Target: Low blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.