Catalog No: OKEH02569
Size:96 Wells
Price: $700.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology 25-OH Vitamin D ELISA Kit (OKEH02569) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-25-OH Vitamin D antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 25-OH Vitamin D and incubated. The 25-OH Vitamin D found in the sample or standards competes with the biotinylated 25-OH Vitamin D for limited binding sites on the immobilized anti-25-OH Vitamin D antibody. Excess unbound biotinylated 25-OH Vitamin D and sample or standard 25-OH Vitamin D is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 25-OH Vitamin D captured in the well and inversely proportional to the amount of 25-OH Vitamin D which was contained in the sample or standard.
ELISA Kit Range3.12 - 200nmol/L
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
Anti-25-OH Vitamin D Microplate96 Wells (12 x 8 Well strips)
25-OH Vitamin D Lyophilized Standard2 x 200 nmol
Sample Diluent1 x 20 mL
100X 25-OH Vitamin D-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: 25OHVD
Target Name: 25-OH Vitamin D
::Pubchem: 5283731
::Chemical formula: C27H44O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
SpecificityNatural and recombinant General 25-OH Vitamin D

Anaparti, V. et al. Circulating levels of free 25(OH)D increase at the onset of rheumatoid arthritis. PLoS One. 2019 14(9): e0219109. 31557191

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias Symbols25OHVD, 25-OH Vitamin D, 25OHVD, Calcifediol, Calcidiol
Protein Name25-OH Vitamin D
Description of TargetLow blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.
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