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Catalog No: OKCA00159
Size:96 Wells
Price: $529.00
SKU
OKCA00159
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:1089%83-96%
Serum (n=4)1:2090%85-95%
Serum (n=4)1:4095%90-99%
Serum (n=4)1:8099%96-104%
ELISA Kit PrincipleAviva Systems Biology 1,25-dihydroxyvitamin D3 ELISA Kit (Mouse) (OKCA00159) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse 1,25-dihydroxyvitamin D3 and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to 1,25-dihydroxyvitamin D3 and incubated. 1,25-dihydroxyvitamin D3 immobilized in the wells competes with the liquid phase 1,25-dihydroxyvitamin D3 contained in the samples or standards for binding with the HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody. Wells are washed removing unbound 1,25-dihydroxyvitamin D3, unbound HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody or 1,25-dihydroxyvitamin D3/HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody complex. HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody bound to 1,25-dihydroxyvitamin D3 immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody captured in the well and inversely proportional to the amount of 1,25-dihydroxyvitamin D3 which was contained in the sample or standard.
ELISA Kit Range1,000 fmol/L - 5,000 fmol/L
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (fmol/L)1996.762052.862097.572033.221934.692016.14
SD0.0730.0760.0550.0760.0810.085
CV%5.6476.0184.3975.9746.1976.669
ELISA Kit Component
ComponentAmount
Anti-1,25-dihydroxyvitamin D3 Microplate96 Well (12 x 8 Well Strips)
1,25-dihydroxyvitamin D3 Standard 5 x 500 uL
High Value Control1 x 500 uL
Middle Value Control1 x 500 uL
Sample Diluent1 x 50 mL
Anti-1,25-dihydroxyvitamin D3 Antibody HRP Conjugate1 x 7 mL
20X Wash Buffer1 x 30 mL
Substrate A1 x 7 mL
Substrate B 1 x 7 mL
Stop Solution1 x 7 mL
Additional Information

CAS#: 32222-06-3

Molecular Formula: C27H44O3

Molecular Weight: 416.63646 g/mol

Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum
Sensitivity< 250 fmoL/L
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse
DHVD 3. No significant cross-reactivity or interference between mouse DHVD 3
and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene Symbol1,25-dihydroxyvitamin D3
Alias SymbolsCalcitriol, 1,25 DHVD3, 1,25-dihydroxyvitamin D3
Protein Name1,25-Dihydroxy vitamin D3
Description of Target1,25-Dihydroxyvitamin D3 is the physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
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