PDE4D ELISA Kit (Human) (OKCD01877)

Catalog No: OKCD01877 (Formerly GWB-KBBFO3)

Size:96WELLS

Human PDE4D ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4D, cAMP Specific (PDE4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphodiesterase 4D, cAMP Specific (PDE4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PDE4D Microplate: 96 Wells (12 x 8 Well Strips)
  • PDE4D Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PDE4D Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 4D, cAMP Specific (PDE4D). No significant cross-reactivity or interference between Phosphodiesterase 4D, cAMP Specific (PDE4D) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PDE4D
• Gene Full Name: phosphodiesterase 4D
• Alias Symbols: ACRDYS2;cAMP-specific 3',5'-cyclic phosphodiesterase 4D;cAMP-specific phosphodiesterase PDE4D6;DPDE3;HSPDE4D;PDE43;PDE4DN2;phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila);STRK1;testicular tissue protein Li 136.
• NCBI Gene Id: 5144
• Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• Description of Target: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
• Uniprot ID: Q08499
• Protein Accession #: NP_001098101.1
• Nucleotide Accession #: NM_001104631.1
• Protein Size (# AA): 809