IFI27 ELISA Kit (Human) (OKCA00702)

Catalog No: OKCA00702

Size:96 Wells

Human IFI27 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	95	90-99
  1:2	86	82-93
  1:4	94	89-98
  1:8	92	85-97

• ELISA Kit Principle: Aviva Systems Biology IFI27 ELISA Kit (Human) (OKCA00702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFI27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFI27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IFI27 captured in the well.
• ELISA Kit Range: 28 pg/mL-1,800 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87	84-90
  EDTA Plasma (n=4)	99	95-102

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IFI27 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IFI27 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • IFI27 Microplate: 96 Wells (12 x 8 Well strips)
  • IFI27 Lyophilized Standard: 2
  • 100X Biotinylated IFI27 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 7 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IFI27
• Gene Full Name: Interferon alpha inducible protein 27
• Alias Symbols: P27, ISG12, FAM14D, ISG12A
• NCBI Gene Id: 3429
• Protein Name: Interferon alpha-inducible protein 27, mitochondrial
• Description of Target: Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
• Uniprot ID: P40305
• Protein Accession #: NP_001123552.1
• Nucleotide Accession #: NM_001130080.2