DSP ELISA Kit (Human) (OKCD01651)

Catalog No: OKCD01651 (Formerly GWB-KBBPB8)

Size:96WELLS

Human DSP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoplakin (DSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoplakin (DSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoplakin (DSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Desmoplakin (DSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmoplakin (DSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmoplakin (DSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DSP Microplate: 96 Wells (12 x 8 Well Strips)
  • DSP Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DSP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Desmoplakin (DSP). No significant cross-reactivity or interference between Desmoplakin (DSP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DSP
• Gene Full Name: desmoplakin
• Alias Symbols: 250/210 kDa paraneoplastic pemphigus antigen;DCWHKTA;desmoplakin;DP.
• NCBI Gene Id: 1832
• Protein Name: Desmoplakin
• Description of Target: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
• Uniprot ID: P15924
• Protein Accession #: NP_001008844.1
• Nucleotide Accession #: NM_001008844.2
• Protein Size (# AA): 2871