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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OAPC00049 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid100 ul culture supernatant. Adequate amount of material to conduct 10-mini Western blots |
Clonality | Monoclonal |
Clone | 235-1 |
Isotype | IgG1 |
Host | Mouse |
Application | IHC, IF |
Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Specificity | Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections. |
Application Info | IF/IHC: 1:100 |
Reference | Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. |
Quality Control | Immunofluorescence performed on each lot |
Description of Target | This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. |
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Molecular Weight | 80kDa & 70kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Human Nuclei (OAPC00049)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Anti-Human Nuclei (OAPC00049)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Human Nuclei (OAPC00049)" provided in?
This item is provided in "Liquid100 ul culture supernatant. Adequate amount of material to conduct 10-mini Western blots".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Human Nuclei (OAPC00049)"?
This target may also be called "" in publications.
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What is the shipping cost for "Anti-Human Nuclei (OAPC00049)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Human Nuclei (OAPC00049)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Human Nuclei (OAPC00049)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "80kDa & 70kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Human Nuclei (OAPC00049)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.